Volume Ii Part 31 (2/2)

I wrote to him [Dr. H. Cruger, of Trinidad] to ask him to observe what the insects did in the flowers of Melastomaceae: he says not proper season yet, but that on one species a small bee seemed busy about the horn-like appendages to the anthers. It will be too good luck if my study of the flowers in the greenhouse has led me to right interpretation of these appendages.

LETTER 628. TO J.D. HOOKER. Down, November 28th [1871].

If you had come here on Sunday I should have asked you whether you could give me seed or seedlings of any Melastomad which would flower soon to experiment on! I wrote also to J. Scott to ask if he could give me seed.

Several years ago I raised a lot of seedlings of a Melastomad greenhouse bush (Monochaetus or some such name) (628/1. Monochaetum.) from stigmas fertilised separately by the two kinds of pollen, and the seedlings differed remarkably in size, and whilst young, in appearance; and I never knew what to think of the case (so you must not use it), and have always wished to try again, but they are troublesome beasts to fertilise.

On the other hand I could detect no difference in the product from the two coloured anthers of Clarkia. (628/2. Clarkia has eight stamens divided into two groups which differ in the colour of the anthers.) If you want to know further particulars of my experiments on Monochaetum (?) and Clarkia, I will hunt for my notes. You ask about difference in pollen in the same species. All dimorphic and trimorphic plants present such difference in function and in size. Lythrum and the trimorphic Oxalis are the most wonderful cases. The pollen of the closed imperfect cleistogamic flowers differ in the transparency of the integument, and I think in size. The latter point I could ascertain from my notes. The pollen or female organs must differ in almost every individual in some manner; otherwise the pollen of varieties and even distinct individuals of same varieties would not be so prepotent over the individual plant's own pollen. Here follows a case of individual differences in function of pollen or ovules or both. Some few individuals of Reseda odorata and R.

lutea cannot be fertilised, or only very rarely, by pollen of the same plant, but can by pollen of any other individual. I chanced to have two plants of R. odorata in this state; so I crossed them and raised five seedlings, all of which were self sterile and all perfectly fertile with pollen of any other individual mignonette. So I made a self sterile race! I do not know whether these are the kinds of facts which you require.

Think whether you can help me to seed or better seedlings (not cuttings) of any Melastomad.

LETTER 629. TO F. MULLER. Down, March 20th, 1881.

I have received the seeds and your most interesting letter of February 7th. The seeds shall be sown, and I shall like to see the plants sleeping; but I doubt whether I shall make any more detailed observations on this subject, as, now that I feel very old, I require the stimulus of some novelty to make me work. This stimulus you have amply given me in your remarkable view of the meaning of the two-coloured stamens in many flowers. I was so much struck with this fact with Lythrum, that I began experimenting on some Melastomaceae, which have two sets of extremely differently coloured anthers. After reading your letter I turned to my notes (made 20 years ago!) to see whether they would support or contradict your suggestion. I cannot tell yet, but I have come across one very remarkable result, that seedlings from the crimson anthers were not 11/20ths of the size of seedlings from the yellow anthers of the same flowers. Fewer good seeds were produced by the crimson pollen. I concluded that the shorter stamens were aborting, and that the pollen was not good. (629/1. ”Shorter stamens”

seems to be a slip of the pen for ”longer,”--unless the observations were made on some genus in which the structure is unusual.) The mature pollen is incoherent, and must be [word illegible] against the visiting insect's body. I remembered this, and I find it said in my EARLY notes that bees would never visit the flowers for pollen. This made me afterwards write to the late Dr. Cruger in the West Indies, and he observed for me the flowers, and saw bees pressing the anthers with their mandibles from the base upwards, and this forced a worm-like thread of pollen from the terminal pore, and this pollen the bees collected with their hind legs. So that the Melastomads are not opposed to your views.

I am now working on the habits of worms, and it tires me much to change my subject; so I will lay on one side your letter and my notes, until I have a week's leisure, and will then see whether my facts bear on your views. I will then send a letter to ”Nature” or to the Linn. Soc., with the extract of your letter (and this ought to appear in any case), with my own observations, if they appear worth publis.h.i.+ng. The subject had gone out of my mind, but I now remember thinking that the imperfect action of the crimson stamens might throw light on hybridism. If this pollen is developed, according to your view, for the sake of attracting insects, it might act imperfectly, as well as if the stamens were becoming rudimentary. (629/2. As far as it is possible to understand the earlier letters it seems that the pollen of the shorter stamens, which are adapted for attracting insects, is the most effective.) I do not know whether I have made myself intelligible.

LETTER 630. TO W. THISELTON-DYER. Down, March 21st [1881].

I have had a letter from Fritz Muller suggesting a novel and very curious explanation of certain plants producing two sets of anthers of different colour. This has set me on fire to renew the laborious experiments which I made on this subject, now 20 years ago. Now, will you be so kind as to turn in your much worked and much holding head, whether you can think of any plants, especially annuals, producing 2 such sets of anthers. I believe that this is the case with Clarkia elegans, and I have just written to Thompson for seeds. The Lythraceae must be excluded, as these are heterostyled.

I have got seeds from Dr. King of some Melastomaceae, and will write to Veitch to see if I can get the Melastomaceous genera Monochaetum and Heterocentron or some such name, on which I before experimented. Now, if you can aid me, I know that you will; but if you cannot, do not write and trouble yourself.

2.X.III. CORRESPONDENCE WITH JOHN SCOTT, 1862-1871.

”If he had leisure he would make a wonderful observer, to my judgment; I have come across no one like him.”--Letter to J.D. Hooker, May 29th [1863].

(631/1. The following group of letters to John Scott, of whom some account is given elsewhere (Volume I., Letters 150 and 151, and Index.) deal chiefly with experimental work in the fertilisation of flowers. In addition to their scientific importance, several of the letters are of special interest as ill.u.s.trating the encouragement and friendly a.s.sistance which Darwin gave to his correspondent.)

LETTER 631. JOHN SCOTT TO CHARLES DARWIN. Edinburgh Botanic Gardens, November 11th, 1862.

I take the liberty of addressing you for the purpose of directing your attention to an error in one of your ingenious explanations of the structural adaptations of the Orchidaceae in your late work. This occurs in the genus Acropera, two species of which you a.s.sume to be unis.e.xual, and so far as known represented by male individuals only. Theoretically you have no doubt a.s.signed good grounds for this view; nevertheless, experimental observations that I am now making have already convinced me of its fallacy. And I thus hurriedly, and as you may think prematurely, direct your attention to it, before I have seen the final result of my own experiment, that you might have the longer time for reconsidering the structure of this genus for another edition of your interesting book, if indeed it be not already called for. I am furthermore induced to communicate the results of my yet imperfect experiments in the belief that the actuating principle of your late work is the elicitation of truth, and that you will gladly avail yourself of this even at the sacrifice of much ingenious theoretical argumentation.

Since I have had an opportunity of perusing your work on orchid fertilisation, my attention has been particularly directed to the curiously constructed floral organs of Acropera. I unfortunately have as yet had only a few flowers for experimental enquiry, otherwise my remarks might have been clearer and more satisfactory. Such as they are, however, I respectfully lay [them] before you, with a full a.s.surance of their veracity, and I sincerely trust that as such you will receive them.

Your observations seem to have been chiefly directed to the A. luteola, mine to the A. Loddigesii, which, however, as you remark, is in a very similar constructural condition with the former; having the same narrow stigmatic chamber, abnormally developed placenta, etc. In regard to the former point--contraction of stigmatic chamber--I may remark that it does not appear to be absolutely necessary that the pollen-ma.s.ses penetrate this chamber for effecting fecundation. Thus a raceme was produced upon a plant of A. Loddigesii in the Botanic Gardens here lately; upon this I left only six flowers. These I attempted to fertilise, but with two only of the six have I been successful: I succeeded in forcing a single pollen-ma.s.s into the stigmatic chamber of one of the latter, but I failed to do this on the other; however, by inserting a portion of a pedicel with a pollinium attached, I caused the latter to adhere, with a gentle press, to the mouth of the stigmatic chamber. Both of these, as I have already remarked, are nevertheless fertilised; one of them I have cut off for examination, and its condition I will presently describe; the other is still upon the plant, and promises fair to attain maturity. In regard to the other four flowers, I may remark that though similarly fertilised--part having pollinia inserted, others merely attached--they all withered and dropped off without the least swelling of the ovary. Can it be, then, that this is really an [andro-monoecious] species?--part of the flowers male, others truly hermaphrodite.

In making longitudinal sections of the fertilised ovary before mentioned, I found the basal portion entirely dest.i.tute of ovules, their place being subst.i.tuted by transparent cellular ramification of the placentae. As I traced the placentae upwards, the ovules appeared, becoming gradually more abundant towards its apex. A transverse section near the apex of the ovary, however, still exhibited a more than ordinary placental development--i.e. [congenitally?] considered--each end giving off two branches, which meet each other in the centre of the ovary, the ovules being irregularly and sparingly disposed upon their surfaces.

In regard to the mere question of fertilisation, then, I am perfectly satisfied, but there are other points which require further elucidation.

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